| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_K611049_sequence (Version 1)
|
Description
Parts K614021 through K614029 are variants of the wild type Tet repressible promoter, R0040. These parts were generated through three rounds of mutagenic PCR. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs. These parts were characterized using K611012.
Notes
This part does not have a termination sequence at the end. It is recommended that for optimal results, this part be used in a backbone that includes a terminator (such as pSB1C3).
Source
Mutagenic PCR of the Tet repressible promoter, R0040.
