Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Timothy Fenton
Date created: 2012-08-20 11:00:00
Date modified: 2015-05-08 01:12:54
LVA∆
5'11 (BBa_K611072) with a +1 deletion (TSS deletion)
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K611084_sequence (Version 1) |
Description
This is the LVA∆5'11 (BBa_K611072) test construct with a transcriptional start site deletion. This mutant was made in order to characterize the promoter activity associated with the LVA-TAATAA-barcode region of BBa_C0051.Notes
noneSource
Parts Registry| Sequence Annotation | Location | Component / Role(s) |
| SpeI BBa_J61002 BBa_B0034 RBS mRFP BBa_B0015 rrnB terminator NotI PstI Verification reverse (VR) primer binding site ColE1 origin pBR322 origin beta-lactamase ampicillin resistance marker beta-lactamase (ampR, bla) promoter Verification forward (VF2) primer binding site BioBrick prefix Ambiguity; should be a T LVA 5 BBa_K611095 Barcode | 1,6 1,2947 8,19 26,706 740,868 751,794 877,884 884,889 947,966 1048,1730 1051,1670 1825,2685 2727,2755 2808,2827 2926,2947 2940,2940 2956,2977 2956,3008 2984,3008 | sequence_feature feature/dna engineered_region feature/BioBrick engineered_region feature/BioBrick CDS feature/cds feature/BioBrick engineered_region feature/stem_loop stem_loop feature/dna sequence_feature feature/dna sequence_feature feature/primer_binding primer_binding_site feature/dna sequence_feature feature/dna sequence_feature feature/cds CDS feature/promoter promoter feature/primer_binding primer_binding_site feature/dna sequence_feature feature/mutation sequence_alteration tag feature/tag engineered_region feature/BioBrick engineered_tag feature/barcode |