BBa_K613000

BBa_K613000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K613000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nadine Guenat, Henrike Niederholtmeyer

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:54

Medium-strength Plac promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K613000_sequence (Version 1)

Description

This is a medium-strong Plac promoter. Placed before a gene, it promotes its expression. Plac is repressed by LacI.
This promoter can be used for in vivo reporter systems; its medium strength is interesting for reporter genes that have a long half-life or that are not well supported by the cell.

Notes

The biobrick sequence does include the -10 and -35 regions; however it doesn't include the ribosome binding site.

Source

This part is the UV5 Lac promoter. The UV5 mutation is a double mutation concerning the -10 region of the promoter, which makes the consensus the -10 region of E.coli (change of TATGTT to TATAAT). The -35 region is not consensus sequence: it sequence is TTTACA, when the consensus would be TTGAC. which makes it a medium strenght promoter.

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K613000/1

Member of these Collections

• iGEM Parts Registry