Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alessandro Ferrari, Vincent Zimmern, Nadine Guenat, Henrike Niederholtmeyer
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:54
T7 promoter family member
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K613002_sequence (Version 1) |
Description
Parts K613001 through K613006 are T7 promoter variants. K613001 is the T7 promoter wildtype. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct.Notes
Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419???10430 (2000).Source
synthetic| Sequence Annotation | Location | Component / Role(s) |
| RBS T7 promoter | 96,101 30,52 | ribosome_entry_site feature/rbs feature/promoter promoter |