BBa_K613014

BBa_K613014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K613014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lilia Salimova

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:54

TetR V36FW43S mutant

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K613014_sequence (Version 1)

Description

This is a TetR mutant, who carries the VF36WS43 mutation.

Notes

The mutation was introduced by site-directed mutagenesis.

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36FW43S mutation by site-directed mutagenesis. The V36F mutation had already been described by Krueger et al (2007).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
V to F	106,108	sequence_alteration, feature/mutation
W to S	127,129	feature/mutation, sequence_alteration

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K613014/1

Member of these Collections

• iGEM Parts Registry