BBa_K613020

BBa_K613020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K613020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nadine Guenat

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:54

TetR P39Q Y42M L197S mutant

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K613020_sequence (Version 1)

Description

This is a TetR mutant with a PQ39YM42LS197 triple mutation.

Notes

The mutation was introduced by site-directed mutagenesis

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36F mutation by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P39Q	115,117	sequence_alteration, feature/mutation
Y42M	124,126	feature/mutation, sequence_alteration
L197S	589,591	feature/mutation, sequence_alteration

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K613020/1

Member of these Collections

• iGEM Parts Registry