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BBa_K613021

BBa_K613021 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K613021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nadine Guenat

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:54

TetR PQ39YM42LP52 mutant

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Details

Types	DnaRegion
Roles	CDS Coding
Sequences	BBa_K613021_sequence (Version 1)

Description

This is a TetR mutant with the PQ39YM42LP52 triple mutation

Notes

The mutation was introduced by site-directed mutagenesis

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced mutations by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P39Q Y42M L52P	115,117 124,126 154,156	sequence_alteration feature/mutation feature/mutation sequence_alteration feature/mutation sequence_alteration