Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Bate, Ben Park, Marc Ammerlaan
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:54
pBAD ompA-zif268
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K618112_sequence (Version 1) |
Description
This is a fusion between ompA and the DNA binding protein zif268 under the control of pBAD. The protein should be present on the outer surface of cells and bind the zif268 recognition sequence (GATGCTGCA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.Notes
NOTE- the sequence as listed here is wrong. Because part K165006 is supplied on a Silver lab vector (Bba_J63010) with a modified biobrick prefix, the scar between ompA and zif268 is non-standard. The G at position 840 is not there. This has been verified by sequencing. Deleting the G keeps zif268 in frame with ompA. Part length is 1364bp.The sequence was generated automatically and I couldn't figure out how to edit it.
Source
parts come from iGEM distribution and from iGEM HQ| Access | Instance | Definition |
| public public public | BBa_K259007 BBa_K103006 BBa_K165006 | BBa_K259007 OmpA BBa_K165006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K259007 BBa_K103006 BBa_K165006 | 1,360 369,832 841,1092 | BBa_K259007 OmpA BBa_K165006 |