Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ben Park, Chris Bate, Marc Ammerlaan
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:54
pLAC ompA-Gli1
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K618211_sequence (Version 1) |
Description
This is a fusion between ompA and the DNA binding protein Gli-1 under the control of pLAC. The protein should be present on the outer surface of cells and bind the Gli-1 recognition sequence (GACCACCCAAGACGA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.Notes
NOTE- the sequence as listed here is wrong. Because part K165007 is supplied on a Silver lab vector (Bba_J63009 or V0002) with a modified biobrick prefix, the scar between ompA and Gli-1 is non-standard. The G at position 840 is not there. This has been verified by sequencing. Deleting the G keeps Gli-1 in frame with ompA. Part length is 1364bp.The sequence was generated automatically and I couldn't figure out how to edit it.
Source
parts come from iGEM distribution and from iGEM HQ| Access | Instance | Definition |
| public public public | BBa_J04500 BBa_K103006 BBa_K165007 | BBa_J04500 OmpA BBa_K165007 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K103006 BBa_K165007 | 1,220 229,692 701,1225 | BBa_J04500 OmpA BBa_K165007 |