| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K619889_sequence (Version 1)
|
Description
This part contains prfA-UTR, an RNA thermosensor from Listeria monocytogenes originally characterized by Johansson et al. Cell 110:551561. The secondary structure of this RNA forms at hairpin at 30'C which masks the ribosomal binding site and initiating ATG. At 37'C this hairpin melts allowing ribosomes to bind and translation to proceed. Be sure that your gene fusion is in frame since the initiating ATG for translation is encoded in this DNA (see diagram from paper). Sequence just before initiating ATG is GAGACATG where the ATG in this sequence is the initiating ATG.
Source
The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.
