| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K619890_sequence (Version 1)
|
Description
This is the prfA-UTR from Listeria monocytogenes which has been randomly mutated to produce a thermosensor this a more discrete dynamic range. In contrast to the our wild type thermosensor (part BBa_K619889) which activates translation from a 30 to 37 degree shift, this thermosensor activates transcription from a 35 to 37 degree shift. This mutation was isolated by randomly mutagenizing part BBa_K619889 and monitoring a lacZ output.
Notes
see BBa_K619889, the Shine-Delagarno was mutated from GGGGGA to GGAGGA in order to optimize expression in E. coli, otherwise the thermosensor is the original sequence of prfA-UTR from Listeria monocytogenes.
Source
This is the prfA-UTR from Listeria monocytogenes.
