| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_K619894_sequence (Version 1)
|
Description
This is the prfA-UTR thermosensor mutated to produce a thermosensor with a increased expression at both 30 and 37 degress when compared with wild-type. One can still see induction when switching from 30 to 37 degrees. Characterization of its dynamic range is underway. The part was made by TAQ-mediated random mutagenesis of our "wild-type" thermosensor (BBa-19889) and subsequent screening using a lacZ reporter. There are other thermosensors entered into the IGEM database with different dynamic ranges obtained from this screen. To use this thermosensor, be sure to clone in frame with the indicated initiating ATG.
Notes
Our "wild-type" thermosensor (BBa-19889) Shine-Delagarno has been optimized for expression in E. coli. Otherwise, the "wild-type" sequence is identical to the thermosensor sequence from Listeria monocytogenes.
Source
prfA-UTR from Listeria monocytogenes
