Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caltech iGEM 2011
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:55
DDT Dehydrochlorinase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K620000_sequence (Version 1) |
Description
Also known as Glutathione S-transferase 1-1, this protein is supposed to degrade DDT, an endocrine disruptor and persistent organic pollutant.Notes
The part was assembled through PCR using a set of long oligos. The sequence contains an NdeI site, which made it more difficult to place into a pET11a vector for protein purification and characterization.Source
This part was isolated from Anopheles dirus. The team took the amino acid sequence found in the NCBI protein database and used reverse transcribed using codon optimization for E. coli to create the DNA part.| Sequence Annotation | Location | Component / Role(s) |
| double stop codon K620000 | 628,633 1,633 | feature/stop stop_codon feature/BioBrick engineered_region |