BBa_K620003

BBa_K620003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K620003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caltech iGEM 2011

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:55

Tet promoter + BisdB

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K620003_sequence (Version 1)

Description

Allows for inducible expression of the protein BisdB, one of a pair of proteins that can degrade bisphenol A (BPA).

Notes

A repeat sequence in the promoter made PCR assembly and Gibson assembly extremely difficult. Ultramers were ordered to prevent mispriming.

Source

Assembled from BBa_R0040, BBa_K123001, BBa_B0014.

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_B0034	BBa_B0034
public	BBa_K123001	BisdB
public	BBa_B0014	BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_B0034	55,66	BBa_B0034
BBa_K123001	67,1350	BisdB
BBa_B0014	1351,1445	BBa_B0014

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K620003/1

Member of these Collections

• iGEM Parts Registry