View

BBa_K624030

BBa_K624030 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K624030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shang-Jui Tsai

Date created: 2011-10-03 11:00:00

Date modified: 2015-05-08 01:12:55

pYMB+[13-R]+[CHAMP-Y]

Back

Details

Types	DnaRegion
Roles	Generator engineered_region
Sequences	BBa_K624030_sequence (Version 1)

Description

pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, 13-R and Y-CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. This design is made in case EYFP fusion on N-terminal of mms13 has negative effects on its folding.

Notes

to fit biobrick assembly standards

Source

BBa_K624004

Components

Access	Instance	Definition
public public public public public	BBa_K624004 BBa_K624012 BBa_K624007 BBa_K624012 BBa_K624025	BBa_K624004 BBa_K624012 BBa_K624007 BBa_K624012 BBa_K624025

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K624004 BBa_K624012 BBa_K624007 BBa_K624012 BBa_K624025	1,1142 1151,1181 1188,2948 2957,2987 2994,3773	BBa_K624004 BBa_K624012 BBa_K624007 BBa_K624012 BBa_K624025