BBa_K625000

BBa_K625000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K625000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Eichenberger
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:56

RBS-LacIM1



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K625000_sequence (Version 1)

Description

Contains a rather weak RBS in front of a codon modified variant of LacI. This reduces the likelihood of recombination with wild type LacI when used in multi plasmid systems.

Notes

The RBS was chosen to match the one in [Basu et al, 2005] in order to create a working band pass filter. Minimal RBS length was chosen to be 40 bp in accordance with [Salis et al, 2009].

Source

PCR on DNA synthesized by GeneArt

Sequence Annotation Location Component / Role(s)
RBS
LacI M1
1,35
36,1121
ribosome_entry_site feature/rbs
CDS feature/cds
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K625000/1