BBa_K625003

BBa_K625003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K625003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Eichenberger
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:56

Pu promoter short version



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K625003_sequence (Version 1)

Description

Contains a shortened version of BBa_K625002 not containing the RBS and first 81bp of XylU but all necessary elements of the promoter.

Notes

All necessary (Valls et al, 2003) elements of the promoter were kept, whereas the first part of XylU and its RBS were deleted.

Source

PCR from the plasmid pCK04AxylR provided by Prof Sven Panke

Sequence Annotation Location Component / Role(s)
XylR binding site
PprA binding site
IHF binding site
-24
-12
XylR binding site
37,52
42,61
130,157
182,188
193,196
69,90
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
feature/promoter promoter
feature/promoter promoter
non_covalent_binding_site feature/binding
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K625003/1