Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Eichenberger
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:56
Minimal pBR322 pMB1 origin
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K625004_sequence (Version 1) |
Description
Origin with low to medium copy number.Notes
Checked all sequences necessary for plasmid replication and defined minimal sequence.Source
PCR on plasmid pSEVA 171 provided by Andreas Bosshard.| Sequence Annotation | Location | Component / Role(s) |
| pMB1 origin ROP ROP RBS RNA II transcript RNA II promoter -35 sequence RNA II promoter -10 sequence RNA I transcript RNA I promoter -35 sequence RNA I promoter -10 sequence | 759,1378 153,344 143,147 772,1324 1355,1360 1334,1339 1214,1321 1178,1183 1200,1205 | sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter promoter feature/promoter feature/misc sequence_feature feature/promoter promoter promoter feature/promoter |