Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM11 Potsdam_Bioware
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:56
mdnA gene encoding the precursor peptide of the tricyclic microviridin
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K627001_sequence (Version 1) |
Description
The BioBrick mdnA is a part of the whole microviridin gene (mdn) cluster, which encodes the protease inhibitor microviridin L. Microviridins are tricyclic depsipeptides, which are ribosomally synthesized by Microcystis aeruginosa (Ziemert et al., 2010). They have a promising potential for therapy as they can block disease-relevant proteases (Ziemert et al., 2008).Microviridins are synthesized from a ribosomal precursor peptide (MdnA). Additionally, the microviridin L biosynthesis gene cluster consists of genes encoding an ATP-grasp-type ligase (mdnB and mdnC) and genes, which encode an ABC transporter (mdnE) and a N-acetyltransferase of the GNAT family (mdnD) (Ziemert et al., 2008).
The following BioBrick mdnA encodes the ribosomal precursor peptide (MdnA), which is essential for microviridin production (Ziemert et al., 2008).
Because this BioBrick is an expression part, the adenin of mdnB gene start codon is part of the XbaI recognition site.
Notes
This BioBrick was built by PCR using the following PCR primersForward primer: TTCCATGGCGCCAGAGGAATCTAGATGGCATATCCCAACGATC
Reverse primer: CTTCTGACTGGGAAGATTATACCGGTTAATACTAGTAGCGGCCGCTGCAGGACGTC
To insert mdnA in the vector pSB1C3, the resulting PCR product and the vector were digested with the restriction enzymes EcoRI and SpeI.