recAp

BBa_K629001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K629001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zilong Wang, Yi ZHENG
Date created: 2011-10-02 11:00:00
Date modified: 2015-05-08 01:12:56

recAp, could be started with exposure to irradiation, UV, nalidixic acid



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K629001_sequence (Version 1)

Description

Name: recAp
+1: 2821841
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 50
Sequence: cttgtggcaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttCaacagaacatattgactatc
Evidence(s): [HIPP] Human inference of promoter position
[TIM] Transcription initiation mapping
Reference(s): [1] Pages V., et al., 2003

Notes

the -35 and -10 district, the RBS

Source

E. Coli BL21 plys genome

Sequence Annotation Location Component / Role(s)
fwd primer
LexA
rev primer
RBS
-10 box
-35 box
1,17
37,56
105,124
106,109
52,60
33,38
primer_binding_site feature/primer_binding
non_covalent_binding_site feature/binding
primer_binding_site feature/primer_binding
feature/rbs ribosome_entry_site
conserved_region feature/conserved
conserved_region feature/conserved
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K629001/1