Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arjun Athreya
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:57
Artificial Yeast Chromosome (Ura3 & Amp)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K635002_sequence (Version 1) |
Description
This part serves as a foundational contribution to expand the registry of yeast components. It is an easy to use artificial yeast chromosome with an auxitrophic uracil selection method for yeast expression, as well as an ampicillin selection for propagation in E.coli. The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest. In addition to the biobrick prefix and suffix, the backbone contains Kpn1 and Sac1 restriction sites the flank the prefix and suffix respectively.Yeast transformants can be selected by using a uracil drop out medium and E.coli transformants may be selected using ampicillin resistance.
Notes
The plasmid backbone contained a Gal1/10 bidirectional promoter between the EcoR1 and BamHI sites on the pRS316 plasmid. The plasmid had to be modified for biobrick compatibility by using the HindIII and SacI sites to digest and ligate a gene fragment with the corresponding HindIII and SacI sites, but which also contained the biobrick prefix and suffix in the correct orientation.Source
The part was modified for biobrick compatibility from a working pRS316 plasmid containing a Gal1/10 bidirectional promoter which was removed through restriction digest.| Sequence Annotation | Location | Component / Role(s) |
| suffix prefix URA3 CEN/ARS Ampicillin resistance psBR322 origin | 1,21 4849,4870 1611,1824 2084,2602 2734,3594 3749,4368 | feature/BioBrick engineered_region feature/BioBrick engineered_region sequence_feature feature/dna feature/misc sequence_feature feature/dna sequence_feature feature/misc sequence_feature |