Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Jones
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:57
Arabinose inducible Reflectin A1-sfGFP fusion generator
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K638301_sequence (Version 1) |
Description
E.coli transformed with this construct will produce a superfolder Green fluorescent Protein (sfGFP) fused variant of reflectin A1 under the control of pBAD, an arabinose inducible protein. There is currently (at the time of writing) no good assay for reflectin, in vivo. This construct allows reflectin to be localised within the cell. This part has been used to view reflectin containing inclusion bodies in E.coli and the sfGFP retained its fluorescence despite the fusion.Notes
We deliberately arranged the sfGFP part of the fusion on the C terminus of the protein as translation is known to proceed from N terminus to C terminus. This means that any sfGFP visualised in vivo must have been produced with reflectin A1 synthesised first, then the sfGFP component.Source
This composite part was built using gibson assembly to add Reflectin A1 between pBAD and sfGFP on an existing pBAD-sfGFP construct.BBa_I0500 - Registry Part
BBa_B0034 - Registry Part
BBa_K638001 - Codon optimised Reflectin A1 Supplied by Wendy Crookes
BBa_I746916 - Registry Part
BBa_B0010 - Registry Part
BBa_B0012 - Registry Part
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 AraC PC Pbad BBa_B0034 conserved reflectin A1 BBa_K638001 BBa_I746916 superfolder GFP coding region start stop BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,1210 1,1079 1043,1114 1080,1210 1219,1230 1223,1226 1237,2299 1237,2299 2299,3015 2299,3015 2299,2301 3013,3015 3030,3109 3041,3084 3111,3151 3118,3137 3138,3151 3144,3144 | engineered_region feature/BioBrick feature/misc sequence_feature promoter feature/promoter feature/promoter promoter engineered_region feature/BioBrick feature/conserved conserved_region feature/cds CDS engineered_region feature/BioBrick feature/BioBrick engineered_region CDS feature/protein start_codon feature/start stop_codon feature/stop engineered_region feature/BioBrick feature/stem_loop stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site stop_codon feature/stop |