BBa_K641002

BBa_K641002 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K641002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Spencer Scott

Date created: 2011-08-30 11:00:00

Date modified: 2015-05-08 01:12:58

{Pctx}{rbs.ffGFP}

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region

Description

This part is a general reporter for any ToxR system. When ToxR dimerizes it binds to Pctx and activates Transcription of genes downstream of Pctx. By putting ffGFP downstream of Pctx the strength of the ToxR construct dimerization is reported by a corresponding strength of GFP expression.

Notes

The only design considerations were tuning an rbs to the ffGFP so that expression levels could clearly be distinguished.

Source

Pctx is a promoter from a virulence island in Vibrio cholerae. The ffGFP is a widely used purely synthetic protein.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K641000	1,144	feature/BioBrick, engineered_region
BBa_K641001	153,182	feature/BioBrick, engineered_region
start	189,191	start_codon, feature/start
superfolder GFP coding region	189,908	CDS, feature/protein
stop	903,908	feature/stop, stop_codon
BBa_I746916	189,908	feature/BioBrick, engineered_region

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K641002/1

Member of these Collections

• iGEM Parts Registry