| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K641005_sequence (Version 1)
|
Description
This is the coding sequence for the ToxR protein that has an nhe1 restriction site before a double gly-ser linker that is used to connect many different types of dimerizing proteins to the N-terminal end of ToxR. This sequence will be the same for most ToxR systems unless changing the linker length. ToxR dimerizes when the protein attached to the N-terminus that sits in the periplasm dimerizes with another ToxR-dimerizing construct.
Notes
There had to be a way to exchange N-terminal parts which is why the Nhe1 site is included after the native sequence of ToxR. Another thing to consider is the length of the linker desired in the system. A GSGS linker was used in our system as the preferred length.
Source
ToxR comes from a virulence island of Vibrio Cholerae.
