Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ian Roney, Matthew Orton
Date created: 2011-10-23 11:00:00
Date modified: 2015-05-08 01:12:58
Constitutive Adh1 distal-Gal1 proximal promoter tagged with yeGFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K642007_sequence (Version 1) |
Description
This is the distal region of the mid length Adh1 promoter (BBa_K319005) submitted last year by the uOttawa team fused to the proximal region of the Gal10/1 promoter. It is a constitutively expressing chimeric promoter. It is also tagged with the reporter yeGFP (BBa_K319039) also submitted by the uOttawa team last year.Notes
The yeGFP part from last year didn't have a stop codon. In PCR amplification of this part, we added a stop codon to the reverse primer.Source
The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal10/1 promoter. The Gal10/1 promoter is commonly used in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.| Sequence Annotation | Location | Component / Role(s) |
| Adh1 distal promoter yeGFP XbaI/SpeI mixed site Gal1 proximal promoter XbaI/SpeI mixed site | 1,516 736,1446 517,522 523,729 730,735 | promoter feature/promoter CDS feature/cds feature/misc sequence_feature feature/promoter promoter feature/misc sequence_feature |