BBa_K643000

BBa_K643000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K643000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sung won Lim

Date created: 2011-09-24 11:00:00

Date modified: 2015-07-21 02:29:26

CDS7 cadmium sulfate binding peptide coding sequence

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K643000_sequence (Version 1)

Description

This sequence codes for a short peptide that's been reported to bind to cadmium sulfate and nucleate quantum dot formation. (C. Mi et al. / Journal of Biotechnology 153 (2011) 125???132)

Peptide sequence: N-GDVHHHGRHGAEHADI-C

Notes

Original coding sequence in the referenced paper had NcoI and BamHI sites surrounding the coding sequence for cloning into pET28b IPTG inducible vector. For our purposes we eliminated the restriction sites and instead replaced them with standard biobricking prefix and suffix.

Source

This part was synthesized de novo based on the information in the original paper. It was PCR'd out of the synthesized plasmid with RFC 23 overhangs and ligated into pSB1C3 vector.

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K643000/1

Member of these Collections

• //binding/metal
• //cds
• iGEM Parts Registry