| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K643004_sequence (Version 1)
|
Description
This is identical in coding sequence to part Bba_K231000 except we wanted to be able to clone this part into the IPTG-inducible expression vector pET28b, so we engineered in restriction sites that would allow us to do so and keep everything in frame. Therefore the part contains an Nco1 site after the Xba1 site, and a BamH1 site before the Spe1 site.
Notes
We wanted to be able to clone this part into the IPTG-inducible expression vector pET28b, so we engineered in restriction sites that would allow us to do so and keep everything in frame. Therefore the part contains an Nco1 site after the Xba1 site, and a BamH1 site before the Spe1 site.
Source
Bba_K231000
