BBa_K648000

BBa_K648000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K648000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lauren Rossi

Date created: 2011-05-26 11:00:00

Date modified: 2015-05-08 01:12:59

mCherry with terminator

Details

• Types: DnaRegion
• Roles: engineered_region; Intermediate
• Sequences: BBa_K648000_sequence (Version 1)

Description

RBS B0034 and truncated mCherry with terminator B0015 attached behind it. mCherry was truncated on the N terminus to remove unnecessary methionine codons.

Notes

the n terminus of mCherry had a sequence which could act as a ribosome binding site near an internal methionine codon which could interfere with the the ribosomes affinity for the actual ribosome binding site. The first 8 amino acids were removed without having any effect on fluorescence.

Source

Parts registry

Components

Access	Instance	Definition
public	BBa_B0034	BBa_B0034
public	BBa_J06505	mCherry
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	1,12	BBa_B0034
BBa_J06505	19,741	mCherry
BBa_B0015	750,878	BBa_B0015

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K648000/1

Member of these Collections

• iGEM Parts Registry