Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jim Rose, Alex Bina, Ben Alouidor, Brian Avison
Date created: 2011-07-02 11:00:00
Date modified: 2015-05-08 01:12:59
Long 10AA Fusion Protein Linker with Standard 25 Prefix/Suffix
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K648006_sequence (Version 1) |
Description
This is the longest of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the ten amino acids GENLYFQSGG and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.Notes
This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly.This part was synthesized through PCR oligo synthesis methods using the following primers:
Forward:
TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCggtgaaaatttgtattttcaa
Reverse:
AATACTGCAGCGGCCGCTACTAGTATTAACCGGTaccaccagattgaaaatacaaattttcacc
Source
Patrick Argos, An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion, Journal of Molecular Biology, Volume 211, Issue 4, 20 February 1990, Pages 943-958, ISSN 0022-2836, DOI: 10.1016/0022-2836(90)90085-Z. (http://www.sciencedirect.com/science/article/pii/002228369090085Z)Ryoichi Arai,Hiroshi Ueda,Atsushi Kitayama,Noriho Kamiya,and Teruyuki Nagamune. Design of the linkers which effectively separate domains of a bifunctional fusion protein Protein Eng. (2001) 14(8): 529-532 doi:10.1093/protein/14.8.529