Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jim Rose
Date created: 2011-07-03 11:00:00
Date modified: 2015-05-08 01:12:59
TEV protease cleaveage site with Standard 25 Prefix/Suffix
| Types | DnaRegion |
| Roles | Protein_Domain polypeptide_domain |
| Sequences | BBa_K648008_sequence (Version 1) |
Description
This is the cleaveage site for th Tobacco etch virus(TEV) protease commonly used for cleaving fusion proteins. It encodes for the amino acids E N L Y F Q G which are cleaved by the TEV protease. This part also contains the prefix/suffix required for standard 25 assembly into fusion parts.Notes
This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly.This part was synthesized through PCR oligo synthesis methods using the following primers:
Forward
5'---ATTAGAATTCGCGGCCGCTTCTAGATGGCCGGCGAGAATTTGTATTTTCAGGG---3'
Reverse
5'---TAATCTGCAGCGGCCGCTACTAGTATTAACCGGTACCCTGAAAATACAAATTCTC---3'
Source
Phan, J., Zdanov, A., Evdokimov, A. G., Tropea, J. E., Peters, H. P. K., Kapust, R. B., Li,M., Wlodawer, A., and Waugh, D. S. (2002). Structural basis for the substrate specificity
of tobacco etch virus protease. J. Biol. Chem. 277: 50564-50572.