BBa_K648009

BBa_K648009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K648009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jim Rose

Date created: 2011-07-03 11:00:00

Date modified: 2015-05-08 01:12:59

RecA Cleavage Site with Standard 25 Prefix/Suffix

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K648009_sequence (Version 1)

Description

This part encodes for the amino acids V Q A G M F S which are cleavage by the RecA protein within the cell. This sequence was taken from the CI repressor protein which is also cleaved by RecA.

Notes

This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly.

This part was synthesized through PCR oligo synthesis methods using the following primers:

Forward Primer

5'---ATTAGAATTCGCGGCCGCTTCTAGATGGCCGGCgttCAGGCAGGGATGTTC---3???


Reverse Primer

5???---TAATCTGCAGCGGCCGCTACTAGTATTAACCGGTtgagaacatccctgcctg ---3???

Source

Kim, B, and Little, LB. "LexA and lambda Cl repressors as enzymes: specific cleavage in an intermolecular reaction.." Cell 73.6 (1993): 1165-73. Web. 4 Jul 2011. .

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K648009/1

Member of these Collections

• //proteindomain/cleavage
• iGEM Parts Registry