BBa_K648011

BBa_K648011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K648011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jim Rose

Date created: 2011-07-03 11:00:00

Date modified: 2015-05-08 01:12:59

Standard 25-Ready Xyle Reporter

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K648011_sequence (Version 1)

Description

This is a mutated version of the Xyle reporter gene which encodes for the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This version of Xyle has been made compatible with standard 25 assembly methods by removing three restriction sites (two NgoMIV sites: at bp 315 and 486, as well as one AgeI site: at bp 837). These mutations were made synonymous with the original sequence and codon optimized for E. Coli.

Because of the synonymous mutations, this gene can be easily used to create fusion protein parts. For more information on the Xyle gene and its uses as a reporter see part BBa_J33204.

Notes

The mutations in this gene were made so that they were synonymous and codon optimized for E. coli cells. The part also contains the prefix/suffix for standard 25 assembly with addition restriction sites NgoMIV and AgeI.

Source

The original genetic material for this part came from part BBa_K316007 which was mutated via PCR based site-directed mutagensis. The xyle portion of this large part was then amplified via PCR reaction and cloned into a new vector along with a prefix/suffix for standard 25 assembly.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
misc	1,918	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K648011/1

Member of these Collections

• //function/reporter/color
• iGEM Parts Registry