BBa_K660300

BBa_K660300 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K660300
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Glasgow Uni 2011
Date created: 2011-09-15 11:00:00
Date modified: 2015-05-08 01:13:03

Phosphodiesterase (enzyme)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K660300_sequence (Version 1)

Description

Phosphodiesterases are a family of enzymes naturally present in microorganisms, which break phosphodiester bonds. The specific enzyme we are using breaks the phosphodiester bond in the second messenger nucleotide, cyclic digaunylate (cyclic-di-GMP). Cyclic-di-GMP is important in many bacterial processes, including biofillm formation and motility. The enzyme regulates signal transduction by controlling levels of the signalling molecule in cells.

We are using the enzyme to control the levels of cyclic-di-GMP within the cell. We expect that the targeted expression of phosphodesiterase could be used to interfere with biofilm formation or to trigger dispersal.

Notes

illegal restriction sites

Source

Amplified from Pseudomonas aeruginosa PA01

Sequence Annotation Location Component / Role(s)
PDE
17,1128
CDS feature/protein
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K660300/1