BBa_K726003

BBa_K726003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K726003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sami Chu

Date created: 2012-08-08 11:00:00

Date modified: 2015-05-08 01:13:05

T7 promoter + lac operator + His tag

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K726003_sequence (Version 1)

Description

T7 promoter is good for protein expression in e. coli. Requires the T7 RNA polymerase. Also contains a RBS, and a His tag for protein purification. Insert your protein of interest behind this.

Notes

none.

Source

The sequence is from pCDFDuet from Novagen.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7 promoter	1,17	feature/promoter, promoter
His tag	100,117	tag, feature/tag
lac operator	22,42	operator, feature/operator

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K726003/1

Member of these Collections

• iGEM Parts Registry