Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: İBRAHİM YASİR ORHAN
Date created: 2012-06-15 11:00:00
Date modified: 2015-05-08 01:13:05
pSB1K3 with RFP coding device
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_K727700_sequence (Version 1) |
Description
We added RFP coding region into the basic pSB1K3 plasmid backbone with aim of using it during our trasnformations. RFP will indicate the false ligations.Notes
Optimized for E.coliSource
Genome of| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 CAP -35 -10 LacI start BBa_B0034 conserved BBa_E1010 mrfp1 barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_J04450 pSB1K3 | 1,200 89,126 137,142 161,166 166,200 173,173 209,220 213,216 227,907 227,901 908,932 941,1020 952,995 1029,1069 1036,1055 1056,1069 1062,1062 1,1069 1079,3273 | engineered_region feature/BioBrick feature/binding non_covalent_binding_site promoter feature/promoter promoter feature/promoter non_covalent_binding_site feature/binding feature/start start_codon feature/BioBrick engineered_region feature/conserved conserved_region feature/BioBrick engineered_region CDS feature/cds engineered_tag feature/barcode engineered_region feature/BioBrick stem_loop feature/stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon engineered_region feature/BioBrick stem_loop feature/stem_loop |