Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Antresh Kumar
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:06
The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide.
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K730001_sequence (Version 1) |
Description
Methylotrophic bacteria are a diverse group of microorganisms with the ability to utilize single-carbon (C1) substrates more reduced than carbon dioxide as their sole source of carbon and energy.Methanotrophs possesses native methanol-inducible promoters, notably promoters which are located upstream of genes that encode methanol dehydrogenase and other proteins required for its activity and enzymes required for the synthesis of the methanol dehydrogenase prosthetic group, pyrroloquinoline quinone. Of these, the promoter PmxaF has been thoroughly scrutinized both biochemically and in expression studies. In its native form in the chromosome, this strong promoter is methanol inducible. However, when this promoter is cloned in expression vectors, it acts essentially in a constitutive mode. The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide. Our system involves the utilization of the methanol as an inducer for MxaF promoter. This would result in the diversion of the flux thus leading to a faster degradation of methane for the cell to survive.Notes
Yes selected 1kb upstream regionSource
GCAAGGTACCGCCGTTCCTCTTCCTGAATCGCCAGTGAGCGTTGGGCCAGCGCACGGTTTTCCTCGCGGGTTTTTTCCAGCGTGCCGACCGTGTGGTTGAAGGCCCTCGAAATGCGGGCG
AATTCCGGCAGGTAGAACTCCGGCAGGCGGCGGCTGTAATCACCGTTTTCCAGGTCTTCC
AGCCCTTCGAGGATGATGCCCACGGATTTGAACGCCCGGCCCAGGGTGGTGTGGACGACC
AGGTACACCGTGACGGCGAGTGCCACCATCAGCAGCAGAAACCCCCGTGCTTCGATCCAG
GCCTCGGCGATCTCGTCGGCCGGGTTGGCGTCAACGGTGATCTGGATCATCGGGTTGCCG
ATCTGGTGCAGATGTTTCTCCGCCACCAGGGGGTCCGGCGCGACGGCCCAGGCGAACCAG
GCGGGCGCTTCCTCACCCTCCGCGGGGCCGGCAGAGGAGGGCAGATCGATGATCGTCCTT
TCGCCTTGATGGATGCTGAGGCTCAGGTGGCGGGCGTGGTCGAGCCGGGCGAGCTGGGCG
ATCCATTCGACCGGCGAGCGACCCGCCGCCACCGCCTGTTCCAGGCCGGCATCGATCAGC
TGTAACGCCAGGTTGACCGATGACCGGACTTCCTCGGCCACCGACCGGCGCGCGTTGTGG
ACCACGAACAGGGTGCCCATGCCGATGATGGCCAGCATGACCAGACCGATCATGAGGTTC
AGGCGAAACCGCAGACTCAAGGGCGCTTGCTCCCGGGAAAGATCGTATTAGTTTGCCTCG
ATCGGCGGTCCTTGTGACAGGGAGATATTCCCGACGGATCCGGGGCATTCGAGCGGAACC
GCCCGCCGTGGGAGTTTTTCCAGCGAGCATTCGAGAGTTTTTCAAGGCGGCTTCGAGGGG
TTATTCCGTAACGCCGCCGACATGATCTGTCCCAGAATCTCCGCCGCTGTTCGTAGAGCG
CCGATGCAGGGTCGGCATCAATCATTCTTGGAGGAGACAC
| Sequence Annotation | Location | Component / Role(s) |
| mxaFpromoter | 1,1000 | feature/promoter promoter |