M256I CysE

BBa_K731010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K731010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jason Fontana, Daniele Rossetto, Giacomo Giacomelli
Date created: 2012-08-19 11:00:00
Date modified: 2015-05-08 01:13:06

M256I CysE (Serine Acetyltransferase)



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K731010_sequence (Version 1)

    Description

    CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.

    This mutated version results to be not inhibited by Cys overproduction, as it secretes Cys.

    Notes

    The construct has a strong RBS (AGGAGG) spaced 8nt from the start codon.

    Source

    PCR extraction from E. coli genome and mutagenesis.

    Sequence Annotation Location Component / Role(s)
    RBS
    Spacer
    Start
    Stop
    		1,6
    7,14
    15,17
    834,839
    		feature/rbs ribosome_entry_site
    sequence_feature feature/misc
    start_codon feature/start
    feature/stop stop_codon

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K731010/1
     

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