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araC-CysEm

BBa_K731030 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K731030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jason Fontana, Daniele Rossetto

Date created: 2012-08-20 11:00:00

Date modified: 2015-05-08 01:13:06

Inducible araC-pBAD promoter regulating M256I CysE

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K731030_sequence (Version 1)

Description

The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731200), which is inducible by arabinose.

Notes

K731200 and K731010 were inserted into pSB1C3 using a variation of the 3A Assembly, which added a PCR amplification step of the inserts before digestion to increase efficiency. The former was cut with E&S, the latter with X&P. pSB1C3 was digested with E&P.

Source

K731200, K731010

Components

Access	Instance	Definition
public public	BBa_K731201 BBa_K731010	BBa_K731201 M256I CysE

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K731201 BBa_K731010	1,1203 1212,2050	BBa_K731201 M256I CysE

Other Properties

igem#experience	Works
igem#partStatus	Released HQ 2013
igem#sampleStatus	In stock
igem#status	Available
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K731030/1