BBa_K731300

BBa_K731300 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K731300
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli
Date created: 2012-08-20 11:00:00
Date modified: 2015-05-08 01:13:06

LacI + LacIq promoter (reverse)



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_K731300_sequence (Version 1)

    Description

    This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG, and it is a very useful device to control and regulate the expression of a gene of interest.

    Notes

    The start codon for LacI is GTG

    Source

    This part comes from the pET-21b plasmid

    Sequence Annotation Location Component / Role(s)
    LacI
    stop
    start
    rbs
    LacIq promoter
    		1,1084
    1,3
    1081,1084
    1085,1092
    1093,1153
    		feature/cds CDS
    feature/stop stop_codon
    feature/start start_codon
    ribosome_entry_site feature/rbs
    feature/promoter promoter

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_K731300/1
     

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