BBa_K731722

BBa_K731722 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K731722
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Depetris, Giacomo Giacomelli
Date created: 2012-08-22 11:00:00
Date modified: 2015-05-08 01:13:06

T1 terminator from E. coli rrnB



    • Details

    Types
    DnaRegion

    Roles
    terminator

    Terminator

    Sequences BBa_K731722_sequence (Version 1)

    Description

    This is the B0010 part extracted by PCR from the doulbe terminator B0012.
    We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use!
    The characterization has been done both with T7 and Coli RNApolymerases.

    Notes

    I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.

    Source

    This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.

    Sequence Annotation Location Component / Role(s)
    B0010
    		1,79
    		feature/misc sequence_feature

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K731722/1
     

    Attachments

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