BBa_K734002

BBa_K734002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K734002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Logan Bachman
Date created: 2012-08-21 11:00:00
Date modified: 2015-05-08 01:13:07

Spinach Aptamer with Stabilizing tRNA Scaffold



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K734002_sequence (Version 1)

Description

The fluorescent Spinach Aptamer (SpA) created by the Jaffrey Lab at Cornell University, designed to bind to the GFP-like synthetic fluorophore, 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). SpA generates a green fluorescence upon binding DFHBI after transcription into mRNA. As reported by the Jaffrey group; excitation wavelength: 469 nm, emission wavelength: 501, with 53% EGFP fluorescent strength.

Source

Generated from SELEX.

Sequence Annotation Location Component / Role(s)
tRNA Scaffold (left)
tRNA Scaffold (right)
Spinach RNA Aptamer
1,30
111,145
31,110
stem_loop feature/stem_loop
stem_loop feature/stem_loop
feature/stem_loop stem_loop
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K734002/1