Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Logan Bachman
Date created: 2012-08-21 11:00:00
Date modified: 2015-05-08 01:13:07
Spinach Aptamer with Stabilizing tRNA Scaffold
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K734002_sequence (Version 1) |
Description
The fluorescent Spinach Aptamer (SpA) created by the Jaffrey Lab at Cornell University, designed to bind to the GFP-like synthetic fluorophore, 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). SpA generates a green fluorescence upon binding DFHBI after transcription into mRNA. As reported by the Jaffrey group; excitation wavelength: 469 nm, emission wavelength: 501, with 53% EGFP fluorescent strength.Source
Generated from SELEX.| Sequence Annotation | Location | Component / Role(s) |
| tRNA Scaffold (left) tRNA Scaffold (right) Spinach RNA Aptamer | 1,30 111,145 31,110 | stem_loop feature/stem_loop stem_loop feature/stem_loop feature/stem_loop stem_loop |