Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Peiran Zhang
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:07
galK is the leader sequence of galactokinase (GalK)
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_K737033_sequence (Version 1) |
Description
galK is the leader sequence of galactokinase (GalK),and Spot42 sRNA controls the synthesis of the galactokinase (GalK) in response to the availability of glucose in the environment,by blocking the recognition of antiSD motif to RBS.The repression is modest(about 2.6 fold),maybe there is no subsequent degradation caused by the major degradosome,RNaesE and RNaseIII.Note:The ClaI site is blocked by GATC methylation due to a mistake.It should be amplified by PCR to get rid of dam methylation before ClaI degestion
Notes
NoSource
Reference:[1] Johannes H. Urban and Jo?? rg Vogel, Translational control and target recognition by Escherichia coli small RNAs in vivo, Nucleic Acids Research, 2007, Vol. 35, No. 3
[2] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008