BBa_K748000

BBa_K748000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K748000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lei Qiao

Date created: 2012-09-14 11:00:00

Date modified: 2015-05-08 01:13:12

AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K748000_sequence (Version 1)

Description

Staphylococcus aureus AgrA, the transcriptional component of a quorum sensing system and global regulator of virulence that up-regulates secreted virulence factors and down-regulates cell wall-associated proteins, can bind in both the P2 and P3 promoter regions of the agr locus. The composite of AgrC and AIP then leads to phosphorylation of AgrA. AgrA in its phosphorylated sate activates transcription from both P2 and P3, leading to the production of GFP and 3OC6HSL. Thus we can detect the presence of S.aureus expediently by observing the expression of GFP.

Notes

As a AIP sensor system, agrA should work with agrC.

Source

Staphylococcus aureus genomic sequence

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K748000/1

Member of these Collections

• //cds/transcriptionalregulator
• //cds/transcriptionalregulator/activator
• //chassis/prokaryote/ecoli
• iGEM Parts Registry