BBa_K754000

BBa_K754000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K754000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Valencia 2012
Date created: 2012-08-27 11:00:00
Date modified: 2015-05-08 01:13:12

S. elongatus PCC7942 psbAI promoter



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K754000_sequence (Version 1)

Description

Our part is the Shynechococcus elongatus promoter PsbA, which in nature downregulates the expression for the Photosystem I components. This promoter works on a constitutive way, althought its activity can be enhanced or decreased in the presence or absence of light (in nature it works regulated in a circadian cicle). This part is suitable for building light-induced devices.

Notes

No special design consideration were taken during the biobrick designing. At any moment was necesarry because this fragment has no tarqet sequences for the standard restriction enzymes used and recomended by the parts registry.

Source

The sequence of this part comes from the AM 2195 plasmid developed by doctor Susan Golden.

Sequence Annotation Location Component / Role(s)
promoter
Functional binding site
AT rich sequence
atypical -10bp element TCTCCT
1,246
191,246
196,216
236,246
feature/promoter promoter
feature/misc sequence_feature
conserved_region feature/conserved
conserved_region feature/conserved
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K754000/1