BBa_K763002

BBa_K763002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K763002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pedro Luis Dorado Morales
Date created: 2012-09-06 11:00:00
Date modified: 2015-05-08 01:13:13

pNirB + Gene encoding ZsGreen1



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_K763002_sequence (Version 1)

    Description

    When our bacteria are growing in absence of oxygen, ZsYellow1 gets expressed. Why?

    FNR, is a DNA binding protein that regulates a large family of genes involved in cellular respiration and carbon metabolism during conditions of anaerobic cell growth. That is because FNR proteins are sensitive to little fluctuations in environmental oxygen concentration (FNR is believed to contain a redox/O2-sensitive element for detecting the anaerobic state). These proteins recognise a DNA target consisting of an inverted repeat: TTGATN1N2N3N4ATCAA (where N 1-4 represents a non-conserved tetrad, NCT).

    Then, when there is no oxygen in the media, FNR proteins bind to promoter and the transcription is activated

    Notes

    No design considerations needed.

    Source

    The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K763002/1
     

    Attachments

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