BBa_K766006

BBa_K766006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K766006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yu Zhao

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:14

AHL activated Plux-LacI

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K766006_sequence (Version 1)

Description

This device is a protein-expression unit which contains a Plux promoter and a transcriptional unit of LacI without LVA-tag. Plux can be activated by luxR-AHL complex and the promoter can activate the expression of downstream genes. We add LacI in this part. As we all know, LacI is a repressor which can bind with Lac operator. With the help of this feature, many logic gates or gene expression modulation can be achieved.

There is not LVA-tag in this part so that the repressor can keep working for a relatively long time.

Notes

We want to design a part that can repress another gene's expression

Source

We get LacI from the plasmid of our lab. Then we use overlap PCR to add the Plux promoter

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Plux promoter	1,55	feature/promoter, promoter
RBS	64,75	feature/rbs, ribosome_entry_site
LacI repressor	82,1170	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K766006/1

Member of these Collections

• iGEM Parts Registry