BBa_K766007

BBa_K766007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K766007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yu Zhao

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:14

Plas and cI hybrid promoter with RFP

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K766007_sequence (Version 1)

Description

This device is composed of a Plas/cI hybrid promoter and a translational unit of RFP. The Plas/cI hybrid promoter is made up of Plas promoter and cI operator. The promoter can be activated by the LasR-C12HSL complex and repressed by cI repressor. This part can be used in building some logic gates.

The RFP segment is used as a signal to show the result of activation or repression. This kind fluorescent protein is very easy to be detected even by eyes.

Notes

We want to design a part that can be activated by one signal but repressed by another signal.

Source

We hybrid Plas promoter and cI repressor by overlap PCR and add RFP downstream by another step of overlap PCR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Plas promoter and cI operator	1,163	feature/promoter, promoter
RBS	172,183	feature/rbs, ribosome_entry_site
RFP	190,870	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K766007/1

Member of these Collections

• iGEM Parts Registry