Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Elitok
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:15
EpCAM Binding Dock: C-terminus
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K772001_sequence (Version 1) |
Description
This gene sequence includes an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease N terminus differently instead of C terminus, in order to induce a signal reaction in the case of EpCAM presence.EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes.
On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
Notes
There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.Source
CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.| Sequence Annotation | Location | Component / Role(s) |
| pCons Promoter RBS OmpA (Dock) TEV Protease C terminus CD215 Antibody Binding Dock | 1,72 73,93 420,857 94,419 858,1159 94,1159 | feature/promoter promoter feature/rbs ribosome_entry_site engineered_region feature/BioBrick feature/BioBrick engineered_region feature/BioBrick engineered_region CDS feature/protein |