Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Elitok
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:15
GFP - LasR protein complex with flag tags
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K772009_sequence (Version 1) |
Description
This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, an inducer regulatory protein (LasR is responsible to induce pLas promoter in the presence of 6HSLO3 which is a chemical compound sythesized by an enzyme that LasI gene codes.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
Notes
Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.Source
Cleavage site, LasR and GFP sequences are gathered from Registry.| Sequence Annotation | Location | Component / Role(s) |
| RBS Flag tag GFP TEV cleavage site LasR Double terminator Anchor-inducer + flag | 1,20 21,46 47,767 768,857 858,1614 1615,1745 21,1614 | feature/rbs ribosome_entry_site tag feature/tag engineered_region feature/BioBrick CDS feature/cds feature/BioBrick engineered_region stop_codon feature/stop feature/protein CDS |