Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Elitok
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:15
Self-Destruction Kit: Endolysin + Holin
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K772101_sequence (Version 1) |
Description
Endolysin is a lysozyme which cleaves the glycosidic bond between the C-1 of N-acetylmuramic acid (NAM) and the C-4 of N-acetylglucosamine (NAG) in the bacterial peptidoglycan (PG). This leads to the complete lysis of the cell.Holin is a protein that assembles together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. It is sourced from T4 bacteriophage.
This composite part can be used to induce self-destruction with a regulatory device in the upstream region.
Notes
There is no RBS in the upstream region, so during the assemble procedure, this must be taken care of.Source
Gene sequences of endolysin and holin are gathered from the Registry.| Access | Instance | Definition |
| public public | BBa_K112806 BBa_K124014 | BBa_K112806 BBa_K124014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K112806 BBa_K124014 | 1,514 523,839 | BBa_K112806 BBa_K124014 |